Hybridoma Screening by Antibody Capture: Detection of Intracellular Binding by Immunofluorescence
Abstract
Hybridoma screening by immunofluorescence stainings of whole cells can be adapted to screen for antibodies to internal proteins by permeabilizing the cells before applying the hybridoma supernatants. In this protocol, cells are attached to a solid support (glass slides), which makes them easy to manipulate and transfer between different reagent solutions.
Footnotes
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From the Antibodies collection, edited by Edward A. Greenfield.
