Recipe

DSP Lysis Buffer

50 mm Tris (pH 8.8)

1 mm EDTA

0.5% Tween 20

200 µg/mL Proteinase K (e.g., Sigma-Aldrich/Roche 3115887001)

Store at room temperature. Add proteinase K immediately before use.

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  1. doi:10.1101/pdb.rec107711 Cold Spring Harb Protoc 2022: pdb.rec107711- © 2022 Cold Spring Harbor Laboratory Press
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