Recipe

Modified Barth's Saline (1×)

5 mm HEPES (free acid)

88 mm NaCl

1 mm CaCl2

1 mm MgSO4

2.5 mm NaHCO3

Prepare as a 10× stock and adjust pH to 7.4–7.8 with NaOH. Store at room temperature.

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  1. doi:10.1101/pdb.rec107713 Cold Spring Harb Protoc 2022: pdb.rec107713- © 2022 Cold Spring Harbor Laboratory Press
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