Topic Introduction

Digoxigenin

  1. Joseph Sambrook

Abstract

In molecular cloning, digoxigenin is used as a ligand that can be incorporated into DNA and RNA probes and detected after hybridization with an anti-digoxigenin-antibody enzyme conjugate. Methods to label nucleic acids with digoxigenin and to detect digoxigenin-labeled probes are introduced here.

Footnotes

  • From the Molecular Cloning collection, edited by Michael R. Green and Joseph Sambrook.

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  1. Published in Advance December 14, 2021, doi: 10.1101/pdb.top100784 Cold Spring Harb Protoc 2022: pdb.top100784- © 2022 Cold Spring Harbor Laboratory Press
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