Table 14.
Selection of immunization protocol
| Protocol | Advantages | Disadvantages |
|---|---|---|
| Classical | Works well for most immunogens | Slower |
| Immunogens with high homology can be difficult. | ||
| RIMMS | Fast | Technically difficult |
| More likely to respond to native form | ||
| cDNA | Good for conformational epitopes and hard-to-express proteins | Technically difficult |
| Not good for intracellular proteins | ||
| Subtractive | Eliminates reactive B and T cells to specific epitopes or proteins | Only 5-wk window before reactivity returns |
| Never 100% effective | ||
| Adoptive transfer | Can increase response to weak immunogens | Technically difficult |
