Table 8.

Suggested doses of immunogens for mice

Form of antigen	Examples	Primary injections and boosts			Final boosts
Form of antigen	Examples	Possible routes^a	Dose	Adjuvant	Possible routes^a	Dose	Adjuvant
Soluble proteins	Enzymes	i.p., s.c.	5–50 µg	+	i.v.	5–50 µg	–
	Carrier proteins conjugated with peptides
	Immune complexes
Particulate proteins	Viruses (killed)	i.p., s.c.	5–50 µg	+	i.v.	5–50 µg	–
	Yeast (killed)
	Bacteria (killed)
	Structural proteins
Insoluble proteins	Bacterially produced from inclusion bodies	i.p., s.c.	5–50 µg	+	i.p.	5–50 µg	–
Insoluble proteins	Immunopurified proteins bound to beads	i.p., s.c.	5–50 µg	+	i.p.	5–50 µg	–
Live cells	Mammalian cells	i.p.	10⁵–10⁷ cells	+/–	i.v.	10⁶ cells	–
Live tumorigenic cells	Oncogenic mammalian cells	i.p., s.c.	10⁴–10⁶ cells	+/–	i.v.	10⁶ cells	–
Carbohydrates	Polysaccharides	i.p., s.c.	10–50 µg	+/–	i.v.	10–50 µg	–
Carbohydrates	Glycoproteins	i.p., s.c.	10–50 µg	+/–	i.v.	10–50 µg	–
Nucleic acids	Carrier proteins conjugated with nucleic acids	i.p.	10–50 µg	+	i.v.	10–50 µg	–