Recipe

Durcupan ACM Epoxy Resin

Component:
A (M epoxy resin; CY 212) 21.6 g
B (Hardener; NY 964) 17.8 g
C (Accelerator 960; DY 060)  1.0 g
D (DBP)  1.9 g
Combine the components in the order given using a disposable plastic container with a tightly fitting lid. By shaking the container, mix vigorously until the mixture is foamy. Next, fill 20–50-mL plastic disposable syringes and seal the syringes using Parafilm. Store the syringes at −20°C. When thawing for use, do not open the syringe until it has reached room temperature to avoid condensation on the cold plastic, which may result in fractures during sectioning.
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  1. doi:10.1101/pdb.rec107836 Cold Spring Harb Protoc 2022: pdb.rec107836- © 2022 Cold Spring Harbor Laboratory Press

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