Protocol

Mosquito Electroantennogram Recordings

  1. Chloé Lahondère1,2,3,4,5,6
  1. 1Department of Biochemistry,
  2. 2The Fralin Life Science Institute,
  3. 3The Global Change Center,
  4. 4Department of Entomology,
  5. 5Center of Emerging, Zoonotic and Arthropod-borne Pathogens, Virginia Polytechnic Institute and State University, Blacksburg, Virginia 24061, USA
  1. 6Correspondence: lahonder{at}vt.edu

Abstract

Electroantennography (EAG) is a simple, yet powerful technique that can be used to indicate that chemical compounds are detected by mosquito antennae. When coupled with gas chromatography (GC), it allows for the identification of detectable compounds within complex mixtures, including those in plant and animal headspaces that might influence mosquito behavior. EAGs and GC-EAGs reveal compounds that can be tested in behavioral assays with the goal of developing new repellents or attractants for mosquito control.

Footnotes

  • From the Mosquitoes collection, edited by Laura B. Duvall and Benjamin J. Matthews.

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  1. Cold Spring Harb Protoc 2022: PROT107871- © 2022 Cold Spring Harbor Laboratory Press
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