Protocol

RNA In Situ Hybridization of Whole-Mount Mosquito Olfactory Tissues

  1. Margaret Herre1,2,3
  1. 1The Laboratory of Neurogenetics and Behavior, The Rockefeller University, New York, New York 10065, USA
  2. 2Kavli Neural Systems Institute, New York, New York 10065, USA
  1. ↵3Correspondence: mherre{at}gmail.com

Abstract

This protocol presents a method for RNA in situ hybridization of whole-mounted preparations of mosquito antennae and maxillary palps. It can be adapted for other peripheral appendages such as the legs or proboscis.

Footnotes

  • From the Mosquitoes collection, edited by Laura B. Duvall and Benjamin J. Matthews.

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  1. Published in Advance August 8, 2022, doi: 10.1101/pdb.prot107916 Cold Spring Harb Protoc 2023: pdb.prot107916- © 2023 Cold Spring Harbor Laboratory Press
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