PBTx for Immunostaining
| Reagent | Amount |
|---|---|
| Deionized H2O | 44.35 mL |
| Phosphate-buffered saline (10×) (e.g., Thermo Fisher AM9625) | 5 mL |
| Dimethyl sulfoxide (e.g., Sigma-Aldrich 472301) | 500 µL |
| Triton X-100 (10%) (e.g., Sigma-Aldrich 93443) | 300 µL |
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Store for up to 1 mo at room temperature.
