Blocking Buffer for Immunostaining
| Reagent | Amount |
|---|---|
| Triton X-100 (10%) (e.g., Sigma-Aldrich 93443) | 4 mL |
| Deionized H2O | 4.5 mL |
| Phosphate-buffered saline (10×) (e.g., Thermo Fisher AM9625) | 1 mL |
| Dimethyl sulfoxide (e.g., Sigma-Aldrich 472301) | 100 µL |
| Normal goat serum (e.g., Jackson ImmunoResearch 005-000-001) | 0.5 mL |
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Prepare fresh.
