Recipe

First Fixative Solution

Reagent Amount Final concentration
Phosphate-buffered saline (10×) (e.g., Thermo Fisher AM9625) 1 mL 1×
Nuclease-free H2O 7 mL
Paraformaldehyde (20%) 2 mL 4%
Triton X-100 (10%) (e.g., Sigma-Aldrich 93443) 30 µL 0.03%

  • Prepare fresh.

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  1. doi:10.1101/pdb.rec107910 Cold Spring Harb Protoc 2023: pdb.rec107910- © 2023 Cold Spring Harbor Laboratory Press
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