Recipe

Tris Buffer 10×

Prepare 0.5 m Tris(hydroxymethyl)aminomethane in milliQ-purified H2O and adjust the pH to 7.4 with 1 m HCl. Check and adjust the pH prior to bringing the working solution up to the final dilution volume. Protect from light and store for up to 1 yr at room temperature.

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  1. doi:10.1101/pdb.rec108230 Cold Spring Harb Protoc 2023: pdb.rec108230- © 2023 Cold Spring Harbor Laboratory Press
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