Lysis Buffer for Western Blotting of Killifish Tissues
10 mm Tris-HCl, pH 8
1% Triton X-100
150 mm NaCl
0.1% SDS
0.5% sodium deoxycholate
0.2% sodium azide
Prepare with milliQ-purified H2O and supplement with protease inhibitors (Roche). Aliquot and store for up to 1 yr at −80°C. Do not refreeze after use.
