Sperm Cryopreservation of the African Turquoise Killifish Nothobranchius furzeri
- Luca Dolfi1,
- Tsz Kin Suen1,
- Danel Medelbekova1,
- Roberto Ripa1,
- Orsolya Symmons1 and
- Adam Antebi1,2,3
- 1Max Planck Institute for Biology of Ageing, 50931 Cologne, Germany
- 2Cologne Excellence Cluster in Cellular Stress Responses in Aging Associated Diseases (CECAD), University of Cologne, 50931 Cologne, Germany
- ↵3Correspondence: aantebi{at}age.mpg.de
Abstract
Sperm cryopreservation is an essential method for the genetic preservation and long-term storage of wild-type and transgenic animal stocks. In addition, it allows for the synchronization of gamete availability and the transport and sharing of lines between different laboratories. Here, we describe a protocol developed in our laboratory for the extraction and cryopreservation of killifish (Nothobranchius furzeri) sperm.
Footnotes
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From the African Turquoise Killifish collection, edited by Anne Brunet.
