In Vitro Fertilization of the African Turquoise Killifish Nothobranchius furzeri
- Luca Dolfi1,
- Tsz Kin Suen1,
- Danel Medelbekova1,
- Roberto Ripa1,
- Orsolya Symmons1 and
- Adam Antebi1,2,3
- 1Max Planck Institute for Biology of Ageing, 50931 Cologne, Germany
- 2Cologne Excellence Cluster on Cellular Stress Responses in Aging Associated Diseases (CECAD), University of Cologne, 50931 Cologne, Germany
- ↵3Correspondence: aantebi{at}age.mpg.de
Abstract
The ability to perform in vitro fertilization, together with sperm cryopreservation, greatly facilitates the long-term laboratory maintenance of wild-type and transgenic model organisms and helps prevent genetic drift. It is also useful in cases where reproduction may be compromised. In this protocol, we present a method for in vitro fertilization of the African Turquoise killifish Nothobranchius furzeri that is compatible with the use of fresh or cryopreserved sperm.
Footnotes
-
From the African Turquoise Killifish collection, edited by Anne Brunet.
