Buffered Sperm-Motility Inhibiting Solution (BSMIS), 1×
75 mm NaCl
70 mm KCl
2 mm CaCl2
1 mm MgSO4
20 mm Tris-Cl, pH 8.0
BSMIS is usually prepared fresh for every sperm freezing session. We note, however, that BSMIS that had been stored for several months at 4°C did work in our hands the few times we used it. We store the component stock solutions for up to a year at room temperature, without a noticeable decline in potency (for our purposes). You may wish to store them at lower temperatures to increase shelf life.
