Embryo Solution
| Reagent | Final concentration or volume |
|---|---|
| Calcium chloride | 0.06 g/L |
| Potassium chloride | 0.0525 g/L |
| Sodium bicarbonate | 0.025 g/L |
| Sodium chloride | 1.125 g/L |
| Methylene blue (commercially available) | 100 µL |
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This is also known as Ringer's solution. The embryo solution can be stored at room temperature.
