Table 1.
Transgenic fly lines used to fluorescently label mitochondria in wing neurons
| Strain | Purpose | BDSC no. | Reference |
|---|---|---|---|
| Drivers | |||
| OK371-Gal4 | GAL4 expression in glutamatergic neurons | 26160 | Meyer and Aberle 2006 |
| Chat-Gal4 | GAL4 expression in cholinergic neurons | 56500 | Salvaterra and Kitamoto 2001 |
| nSyb-Gal4 | GAL4 expression pan-neuronally | 68222 | Verstreken et al. 2009 |
| Appl-Gal4 | GAL4 expression pan-neuronally | 32040 | Torroja et al. 1999 |
| nSyb-lexA | LexA expression pan-neuronally | 52817 | Pfeiffer et al. 2012 |
| Fluorescent markers | |||
| 5xUAS-mito::GFP | Label the mitochondrial inner membrane with GFP | 8442/8443 | Rikhy et al. 2003 |
| 5xUAS-mito::tdTomato | Label the mitochondrial inner membrane with tdTomato | N/A | Smith et al. 2019b |
| 14xUAS-mCherry::mito.OMM | Label the mitochondrial outer membrane with mCherry | 66532/66533 | Vagnoni and Bullock 2016 |
| lexAop-mCherry::mito.OMM | Label the mitochondrial outer membrane with mCherry | 66530/66531 | Vagnoni and Bullock 2016 |
| 5xUAS-mCD8::GFP | Label cell membrane with GFP | 32192 | Lee and Luo 1999 |
| 10xUAS-IVS-myr::tdTomato | Label cell membrane with tdTomato | 32222 | Wong et al. 2002 |
| MARCM machinery | |||
| asenseFLP2e | Mediate homologous recombination between FRT sites to induce MARCM clones | N/A | Neukomm et al. 2014 |
| FRT2A | FLP recognition site on chromosome arm 3L for homologous recombination | 1997 | Golic 1991 |
| FRT82B | FLP recognition site on chromosome arm 3R for homologous recombination | 2035 | Xu and Rubin 1993 |
| tubulin-Gal80 | Represses GAL4 in all cells. Can be removed by FLP-mediated recombination to label a subset of cells. | 5135 | Lee and Luo 1999 |
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(BDSC) Bloomington Drosophila Stock Center, (GFP) green fluorescent protein, (N/A) not currently available from the stock center and must be requested from the laboratory directly, (MARCM) mosaic analysis with a repressible cell marker.
