Single-Pair and Small-Group Crosses of Aedes aegypti
- 1W. Harry Feinstone Department of Molecular Microbiology and Immunology, Johns Hopkins Malaria Research Institute, Johns Hopkins Bloomberg School of Public Health, Johns Hopkins University, Baltimore, Maryland 21205, USA
- 2The Solomon H. Snyder Department of Neuroscience, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA
- ↵3Correspondence: cmcmeni1{at}jhu.edu
Abstract
Aedes aegypti is an emerging model insect species used in genetics studies because of its ease of laboratory rearing, desiccation-resistant eggs, expanding genetic toolkit, and high-quality reference genome. Here, we describe procedures to isolate and sex virgin female and male mosquitoes and establish successful mating crosses. We also detail how to blood feed mosquitoes from these crosses, isolate individual or small groups of females for egg laying, condition these eggs for storage and hatching, and verify female mating status.
Footnotes
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From the Mosquitoes collection, edited by Laura B. Duvall and Benjamin J. Matthews.
