Aedes aegypti Saline Solution for Imaging
| Reagent | Final concentration |
|---|---|
| NaCl | 103 mm |
| KCl | 3 mm |
| TES | 5 mm |
| NaHCO3 | 26 mm |
| NaH2PO4 | 1 mm |
| CaCl2 | 1.5 mm |
| MgCl2 | 4 mm |
| Trehalose | 10 mm |
| Glucose | 10 mm |
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We typically make a 2-L stock in a volumetric flask, adding water and commercial solutions of CaCl2 and MgCl2 before other ingredients to avoid calcium precipitation. Adjust the pH to 7.1 with HCl or NaOH solution using a pH meter (Beyenbach and Masia 2002). Store at 4°C for up to 2 wk. Note that this recipe is based on a standard recipe used for Drosophila melanogaster, with the pH adjusted to a published value for Ae. aegypti (Beyenbach and Masia 2002). It is also likely to be compatible with other mosquitoes.
