Recipe

Marc's Modified Ringer Solution (MMR) (10× stock)

1 m NaCl

20 mm KCl

10 mm MgSO4

20 mm CaCl2

50 mm HEPES (pH 7.5)

Prepare a 10× stock in deionized H2O, adjust the pH to 7.8 with NaOH, and sterilize by autoclaving. Store solution indefinitely at room temperature.

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  1. doi:10.1101/pdb.rec108045 Cold Spring Harb Protoc 2023: pdb.rec108045- © 2023 Cold Spring Harbor Laboratory Press

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