Topic Introduction

Obtaining Xenopus Eggs and Embryos

  1. Richard M. Harland2
  1. 1Northeastern University, Boston, Massachusetts 02115, USA
  2. 2Department of Molecular and Cell Biology, University of California, Berkeley, California 94720-3200, USA
  1. 3Correspondence: h.sive{at}northeastern.edu

Abstract

Collecting eggs from adult Xenopus laevis and Xenopus tropicalis to raise healthy embryos and tadpoles is relatively simple but requires careful handling of the frog. Eggs can be fertilized through natural matings or by in vitro fertilization and examined visually. Here we review how eggs are obtained and how to recognize healthy eggs that will develop into high-quality embryos.

Footnotes

  • From the Xenopus collection, edited by Hazel L. Sive.

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