Recipe

25% Percoll in 1× NPB

2 mL 2× NPB

1 mL Percoll (e.g., Sigma-Aldrich P4937; stored at 4°C)

1 mL sterile Nanopure H2O

Freshly prepare 4 mL and store on ice.

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  1. doi:10.1101/pdb.rec108031 Cold Spring Harb Protoc 2023: pdb.rec108031- © 2023 Cold Spring Harbor Laboratory Press
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