1× NPB with 0.3% BSA

2.5 mL 2× NPB

2.35 mL sterile Nanopure H₂O

150 µL 10% bovine serum albumin (BSA; e.g., Sigma-Aldrich A3294; 10% w/v with pH adjusted to 7.6 with KOH, aliquoted, and stored at −20°C)

Freshly prepare 5 mL and store on ice.

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