Hatching Solution for Killifish
Dissolve 1 g of humic acid (Sigma-Aldrich 53680) in 1 l of reverse-osmosis-treated H2O. Autoclave at 15 psi for 20 min at 121°C. Store the solution at 4°C until use, as hatching efficiency drops when the hatching solution is equilibrated to room temperature before use. The solution can be used for at least 2 mo.
