Killifish Embryo Solution
Dissolve two Ringer's tablets (Millipore 96724) in 1 L of MilliQ-purified H2O or reverse-osmosis-treated H2O. Sterilize the solution with 0.22-µm filters or autoclave the solution (15 psi, 20 min, 121°C). Dilute methylene blue (e.g., Kordon 37344) in the Ringer's solution at a final concentration of 0.002%–0.01% (e.g., 1 L of Ringer's solution with 100 µL of methylene blue). Protect the solution from light, and store at room temperature (good for at least 2 mo).
