Staphylococcus aureus Colony Polymerase Chain Reaction
- 1Microbiology, School of Biological and Chemical Sciences, University of Galway, Galway, H91 TK33, Ireland
- 2Center for Pandemic Vaccines and Therapeutics (ZEPAI), Paul-Ehrlich-Institute, 63225 Langen, Germany
- 3Section of Molecular Microbiology and Medical Research Council Centre for Molecular Bacteriology and Infection, Imperial College London, Exhibition Road, London, SW7 2AZ, United Kingdom
- ↵4Correspondence: a.grundling{at}imperial.ac.uk
Abstract
Here, we describe a protocol for a colony polymerase chain reaction (PCR) method for Staphylococcus aureus. The methodology involves the preparation of small S. aureus lysates by using the enzyme lysostaphin to degrade the peptidoglycan layer. These lysates are prepared using a small patch of bacteria grown on LB agar plates, and the lysates can subsequently be used for PCR analyses.
Footnotes
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From the Experiments in Bacterial Genetics collection, edited by Lionello Bossi, Andrew Camilli, and Angelika Gründling.
