Table 1.
Feeding schedule for rearing Culex larvae at 24°C–27°C
| Pan (50 mg food) | 100 mg food | No feed | 250 mg food | No feed | No feed | 350 mg food | No feed | Pick 1 | Pick 2 | Cage Info | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Date | Number of pans | Initial | Purpose (main colony/apt ID) | Cohort | Date | Initial | Date | Date | Initial | Date | Date | Date | Initial | Date | Date | Initial | Date | Initial | Emergence date/notes |
| 7.25.22 | 12 | AS | Main colony | F42 | 7.26 | MW | 7.27 | 7.28 | AS | 7.29 | 7.30 | 7.31 | MEM | 8.01 | 8.02 | MEM | 8.03 | MW | Emerged 08.02 |
| 8.04.22 | 6 | MW | FlyBox | F43 | 8.05 | MW | 8.06 | 8.07 | MEM | 8.08 | 8.09 | 8.10 | MW | 8.11 | 8.12 | AS | 8.13 | MEM | Placed into small cages |
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The top row indicates how much food should be given or when mosquito pupae should be picked (Pick 1 and 2). Labels for each column represent other important information that should be recorded starting when the pans of larvae are established and each subsequent day thereafter. Researchers use their initials to indicate who fed the mosquito larvae and when, allowing multiple people to easily and accurately care for laboratory colonies.
