Antigen Retrieval Solution

Reagents

HCl (e.g., Merck Life Science 7647-01-0)

Milli-Q H₂O (sterile)

Tri-sodium citrate dihydrate (e.g., Merck Life Science 6132-04-3)

Tween 20 (e.g., Merck Life Science 9005-64-5)

• 1. Add 2.94 g of tri-sodium citrate dihydrate to 1000 mL of sterile Milli-Q H₂O. Shake to dissolve.

• 2. Adjust the pH to 6.0 with HCl.

• 3. Add 0.5 mL of Tween 20.

• 4. Store for up to 3 mo at room temperature or for longer periods (up to 6 mo) at 4°C.

  • This solution is also called sodium citrate buffer (10 mm sodium citrate, 0.05% Tween 20, pH 6.0).