Isolation of XO Subclones from XY Murine Embryonic Stem Cells
- 1Department of Genetics and Development, Columbia University Medical Center, New York, New York 10032, USA
- 2Department of Genetics, University of Texas MD Anderson Cancer Center, Houston, Texas 77030, USA
- ↵3Correspondence: vep1{at}columbia.edu
Abstract
This is a simple procedure to isolate XO subclones from XY murine embryonic stem cells in situations that require transmission of a mutation through the female germline—for example, if the mutation adversely affects spermatogenesis. XY cells are plated at clonal density, and resulting colonies are genotyped by polymerase chain reaction for a Y-specific probe to identify clones that have spontaneously lost the Y chromosome.
Footnotes
-
From the Mouse Phenotypes collection by Virginia E. Papaioannou and Richard R. Behringer.
