Recipe

Isotonic Buffer

150 mm NaCl

5 mm KCl

10 mm HEPES

2.5 mm CaCl2

Adjust pH to 7.2 and filter sterilize with a 0.22-µm filter. Store for 1 mo at 4°C.

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  1. doi:10.1101/pdb.rec108078 Cold Spring Harb Protoc 2024: pdb.rec108078- © 2024 Cold Spring Harbor Laboratory Press
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