Isotonic Buffer
150 mm NaCl
5 mm KCl
10 mm HEPES
2.5 mm CaCl2
Adjust pH to 7.2 and filter sterilize with a 0.22-µm filter. Store for 1 mo at 4°C.
150 mm NaCl
5 mm KCl
10 mm HEPES
2.5 mm CaCl2
Adjust pH to 7.2 and filter sterilize with a 0.22-µm filter. Store for 1 mo at 4°C.