Protein Sample Buffer
| Reagent | Final concentration |
|---|---|
| Tris (pH 6.8) | 50 mm |
| Bromophenol blue | 0.05% (w/v) |
| Sodium dodecyl sulfate (SDS) | 2% (w/v) |
| Glycerol | 10% (v/v) |
| Dithiothreitol (DTT) | 50 mm |
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Prepare protein sample buffer without DTT and store for up to 1 yr at room temperature. Freshly add DTT before use.
