PBSP Buffer
| Reagent | Amount or final concentration |
|---|---|
| Pluronic F108 NF surfactant (BASF) | 10 mL |
| NaCl | 150 mm |
| Na2HPO4 | 8 mm |
| NaH2PO4 | 2 mm |
-
1. Add reagents to sterile H2O.
-
2. Adjust pH to 7.4 using HCl or NaOH.
-
3. Bring the volume to 1 L.
-
4. Filter-sterilize (0.2 µm).
-
Store for up to 2 mo at room temperature; discard if it becomes turbid.
-
