Phosphate-Buffered Saline with Tween (0.1%) (PBST)
| Reagent | Final concentration |
|---|---|
| NaCl | 150 mm |
| Na2HPO4 | 8 mm |
| NaH2PO4 | 2 mm |
| Tween 20 | 0.1% (w/v) |
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Adjust to pH 7.4 with NaOH or HCl and filter-sterilize with a 0.45-µm filter. Store at room temperature.
