Recipe

Agar (1.5%)–Sucrose (2%) Solution

Reagent Vendor Amount
Drosophila agar Genesee Scientific 66-105 1.5% (w/v)
Sucrose solution Fisher Scientific S5-12 2% (w/v)
  • 1. Make 2% sucrose solution by adding sucrose to Milli-Q H2O.

  • 2. Use the 2% sucrose solution to dissolve the agar. A microwave may be used to heat the solution to dissolve further, ensuring not to bring it to a vigorous boil.

Store for up to 10 d at 4°C.

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  1. doi:10.1101/pdb.rec108525 Cold Spring Harb Protoc 2024: pdb.rec108525- © 2024 Cold Spring Harbor Laboratory Press

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