Recipe

PBT Buffer with Block (PBTB)

Dulbecco's phosphate-buffered saline (DPBS; 1×; Wisent)

0.1% (v/v) Tween-20

1% (w/v) bovine serum albumin (Invitrogen)

Freshly prepare; can be stored for up to 1 wk at room temperature.

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  1. doi:10.1101/pdb.rec108485 Cold Spring Harb Protoc 2024: pdb.rec108485- © 2024 Cold Spring Harbor Laboratory Press
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