Antibody Dilution Buffer for Whole-Mount Immunohistochemistry
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1. In a 15-mL Falcon tube, combine 200 mg of bovine serum albumin (final concentration 2%, w/v), 9.56 mL of Dulbecco’s phosphate-buffered saline (DPBS; Wisent), 200 µL of 10% (v/v) normal sheep serum (Jackson ImmunoResearch Inc.), and 40 µL of Triton X-100.
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2. Vortex this mixture well. Makes 10 mL.
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For optimal results, freshly prepare; however, aliquots can be made and stored long term (up to 1 yr) at −20°C.
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