Recipe

Loading Buffer (6×)

Add 1.8 mL of Tris-HCl (1.0 m, pH 6.8) to 1.5 mL of glycerol, 0.6 g SDS, 0.464 g DTT, and 0.15 mL of 1% (w/v) bromophenol blue. Adjust the volume to 5 mL with double distilled H2O and store for up to 1 yr at −20°C.

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  1. doi:10.1101/pdb.rec108491 Cold Spring Harb Protoc 2024: pdb.rec108491- © 2024 Cold Spring Harbor Laboratory Press
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