Recipe

Stacking Gel Mixture (4%)

Tris-HCl (1.0 m, pH 6.8) 0.313 mL
Double-distilled H2O 1.913 mL
Acrylamide/Bis solution 29:1 (30%; Bio-Rad) 0.25 mL
SDS, 10% (w/v) 0.025 mL
Ammonium persulfate, 10% (w/v) 0.025 mL
Tetramethylethylenediamine (TEMED;
 Bio-Rad Laboratories 161-0801) 0.005 mL

Freshly prepare. Add TEMED last, immediately before pouring the gel solution into the gel casting apparatus.

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  1. doi:10.1101/pdb.rec108494 Cold Spring Harb Protoc 2024: pdb.rec108494- © 2024 Cold Spring Harbor Laboratory Press
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