Transfer Buffer (1×)
Prepare 10× transfer buffer as follows:
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22.5 g Tris
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105 g Glycine
Add double-distilled H2O to 1 L. Store at room temperature.
Freshly prepare the 1× working buffer by adding:
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100 mL 10× stock transfer buffer
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200 mL methanol (100%; Sigma-Aldrich)
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700 mL double-distilled H2O
Chill to 4°C before use.
