Recipe

Rinaldini Solution

Reagent Distributor and catalog number Final concentration
NaCl Nacalai Tesque 31319-45 8 mg/mL
KCl Nacalai Tesque 28513-85 0.2 mg/mL
NaH2PO4·H2O Sigma-Aldrich 71507 0.05 mg/mL
NaHCO3 Nacalai Tesque 31212-25 1 mg/mL
Glucose Wako Chem 046-31175 1 mg/mL
Penicillin/streptomycin Thermo Fisher 15140122 1× (100 U/mL)
  • Sterilize solution using a 0.2-µm syringe filter. Prepare in advance and store for up to 2 wk at 4°C or for up to 1 mo at −20°C.

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  1. doi:10.1101/pdb.rec108507 Cold Spring Harb Protoc 2024: pdb.rec108507- © 2024 Cold Spring Harbor Laboratory Press

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