Neuronal Culture Medium 1
| Reagent | Distributor and catalog number | Composition |
|---|---|---|
| Schneider's Drosophila medium | Thermo Fisher 21720024 | 44.5 mL |
| Fetal bovine serum | Thermo Fisher 10082147 | 5 mL (10%, v/v, final) |
| Penicillin/streptomycin | Thermo Fisher 15140122 | 0.5 mL (100 U/mL) |
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Sterilize the solution using a 0.2-µm syringe filter. Prepare the solution in advance and store for up to 2 wk at 4°C or for up to 1 mo at −20°C.
