Recipe

Neuronal Culture Medium 2

Reagent Distributor and catalog number Composition
Drosophila defined medium (DDM2) N/A 30 mL
Neurobasal medium Thermo Fisher 21103049 10 mL
B27 supplement (50×) Thermo Fisher 17504044 0.8 mL
Penicillin/streptomycin Thermo Fisher 15140122 1× (100 U/mL)
20-Hydroxyecdysone (ecdysone) Sigma-Aldrich H5142 20 µm

  • Combine the reagents and sterilize the solution using a 0.2-µm syringe filter. Prepare in advance and store C for up to 2 wk at 4°C or for up to 1 mo at −20°C.

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  1. doi:10.1101/pdb.rec108513 Cold Spring Harb Protoc 2024: pdb.rec108513- © 2024 Cold Spring Harbor Laboratory Press
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