Drosophila Apple Juice-Agar Plates
MATERIALS
Reagents
Agar
Apple juice
Nipagin M (20% in absolute ethanol)
Sucrose
Equipment
Flasks, conical
Jug, plastic, 2-L
Magnetic stirrer and stirring rod
Oven, microwave
Petri dishes, 35- or 90-mm
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This recipe will make 65 35-mm plates or 40 90-mm plates. Use the lid of the 35-mm plates.
METHOD
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1. In a conical flask, mix 18 g of agar in 600 mL of H2O.
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2. In a separate flask, mix 20 g of sucrose in 200 mL of apple juice.
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3. Place both flasks in a microwave oven on a medium setting (e.g., 7 on a scale of 0–10) for 30 min.
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4. When both the agar and sucrose are dissolved, pour the apple juice into the plastic jug with a magnetic stirring rod.
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5. Carefully (to avoid bubbles) add the agar solution to the jug. Place the mixture on a magnetic stirrer. Allow the mixture to cool to at least 60°C.
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6. Add 20 mL of 20% Nipagin M. Stir for a few minutes.
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7. Pour into plates.
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8. Allow the medium to solidify. Store the plates at 4°C.
