Cover image

Obtaining timing and spatial information about the expression of genes of interest is crucial for our understanding of developmental and cell biology. Although multiple techniques are available for evaluating this at the RNA level, determining expression patterns for low-abundance transcripts remains a challenge. The African turquoise killifish Nothobranchius furzeri is a short-lived fish species that has emerged as a powerful model for studies in multiple research areas. In this issue, Philip B. Abitua describes a technique for the visualization of low-copy mRNA in Nothobranchius furzeri embryos using tyramide signal amplification (doi:10.1101/pdb.prot107805). The cover image, provided by the author, shows a killifish embryo probed for tbx1 mRNA and counterstained with DAPI at 4 days postfertilization.