Recipe

DNase I Solution (10 mg/mL)

Add 10 mg of deoxyribonuclease I (DNase I, from bovine pancreas; Sigma-Aldrich DN25) to 1 mL of filtered, autoclaved Milli-Q H2O or tissue-culture-grade H2O.

Store for up to 2 mo at 4°C. DNase I degrades free DNA and prevents cell aggregation.

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  1. doi:10.1101/pdb.rec108277 Cold Spring Harb Protoc 2024: pdb.rec108277- © 2024 Cold Spring Harbor Laboratory Press

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