Recipe

Prehybridization Buffer for Nothobranchius

Reagent stock Volume required for 50 mL Final concentration
Formamide 100% (Sigma-Aldrich
 221198-1L) 		25 mL 50%
SSC 20× 12.5 mL 5×
Tween-20 (VWR 97062-332),
 10% (v/v) stock 		250 µL 0.05%
H2O Adjust to 50 mL

  • Adjust to pH 6.0 with 1 m citric acid. Sterilization is not necessary. Store for up to a year at −20°C.

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  1. doi:10.1101/pdb.rec108311 Cold Spring Harb Protoc 2024: pdb.rec108311- © 2024 Cold Spring Harbor Laboratory Press
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