PBTween (0.05%)
| Reagent stock | Volume required for 1 L | Final concentration |
|---|---|---|
| PBS, 10×, pH 7.4 (Thermo Fisher AM9624) | 100 mL | 1× |
| Tween-20 (VWR 97062-332), 10% (v/v) stock |
5 mL | 0.05% |
| H2O | 895 mL |
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Store indefinitely at room temperature. Bottle vacuum filtration is recommended to prolong shelf life. Discard if a precipitate forms.
